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Kathleen Doane, PhD

Professor of Anatomy
Department of Medical Education
Research & Scholarship
North Campus

LOCATION(S)
Geisinger Commonwealth School of Medicine
Medical Sciences Building
525 Pine St.
Office 2040
Scranton, PA 18509
570-955-1319
Fax: 570-504-9618
kdoane@som.geisinger.edu

Professor of Anatomy

Biography

Kathleen Doane, PhD, received her PhD in cell and developmental biology from Rutgers, The State University of New Jersey and the University of Medicine and Dentistry of New Jersey. She did her postdoctoral work at Tufts Medical School. Her research interests concentrate on the interaction of cells with the extracellular matrix using matrix receptors. Dr. Doane came to Geisinger Commonwealth from Northeastern Ohio Universities College of Medicine.

Research interests

Dr. Doane’s major research interests concentrate on the interaction of cells with the extracellular matrix using matrix receptors. Her major project elucidates the cell-matrix interactions important in heart failure fibroblasts. This NIH funded project (1R15HL079969) is examining the role of collagen VI and matrix receptor signaling in transgenic type VI collagen knockout mice. Interaction of fibroblasts with type VI collagen has been shown to cause these cells to differentiate into myofibroblasts, a wound healing phenotype that is hyperproliferative and hypersecretory. We have demonstrated an increase in type VI collagen following myocardial infarction and subsequent to administration of isoproterenol, a synthetic catecholamine.

An increase in markers for myofibroblasts indicates a possible correlation between the presence of these cells and type VI collagen during a disease/wound healing state. Our hypothesis is that cells subjected to abnormal conditions begin to secrete type VI collagen, which causes excess myofibroblast differentiation. This may lead to excessive fibrosis during wound healing due to the hypersecretion by these cells, and development of a fibrotic scar due to excess proliferation. Control of the deposition of the collagen or blockade of the signaling pathway leading to myofibroblast differentiation could result in new treatment paradigms for heart disease.

Other projects:

Using breast cancer cell lines to study the interaction of integrins and urokinase plasminogen activator receptor at different levels of invasiveness (collaboration with Akron General Medical Center).

Development of a liquid-crystal-based pathogen detection system. There are two licensees and several patents covering this technology (collaboration with Northeastern Ohio Universities College of Medicine, Kent State University and Pathogen Systems Inc.).

Analysis of phenotypic alterations in synovial fibroblasts following metal challenge as a model for development of joint failure following arthroplasty (collaboration with Summa Health Care Systems).

Gene therapy by nanosphere delivery using a kidney hypertension model (collaboration with University of Akron and Walsh University).

Patents:

  • “Detection and Amplification of Ligands” Patent number 6,171,802, awarded 1/9/01
  • “Detection and Amplification of Ligands” Patent number 7,160,736, awarded 2/1/07
  • “Detection and Amplification of Ligands” Patent number 7,267,957, awarded 9/11/07

Our research is supported by a grant from National Heart, Lung, and Blood Institute.

Recent publications

Peer-reviewed publications

  • Moncman CL, Panaviene Z, Norris A, Deng XA, Esham M, Doane KJ, (2010). Deletion of the nebulette linker region stalls myofibril assembly at the peripheral edges of spreading cardiomyocytes. Exp. Cell Res.
  • Nugent AE, Speicher DM, Gradisar I, McBurney DL, Baraga A, Doane KJ, Horton Jr WE, (2009). Advanced osteoarthritis in humans is associated with altered collagen VI expression and up-regulation of ER-stress markers Grp78 and Bag-1. J. Histochem. Cytochem. Full text
  • Bryant, J.E., P.E. Shamhart, D.J. Luther, E.R. Olson, J.C. Koshy, D.J. Costic, M.V. Mohile, M. Dockry, K.J. Doane, J.G. Meszaros (2009) Cardiac myofibroblast differentiation is attenuated by alpha(3) integrin blockade: potential role in post-MI remodeling. J. Mol. Cell Cardiol. 46:186-192. PMID: 19041328. Full text
  • Jenkins, C., A. Milsted, K. Doane, G. Meszaros, J. Toot, D. Ely (2007) A cell culture model using rat coronary artery adventitial fibroblasts to measure collagen production. Cardiovascular Disorders 7:13-22, 2007. PMID: 17488510. Full text
  • Naugle, J.E., E.R. Olson, X. Zhang, S. Mase, K.J. Doane, J.G. Meszaros (2006) Type VI collagen regulation of cardiac remodeling. Am. J. Physiol. Heart Circ. Physiol. 290:H323-H330. PMID: 16143656. Full text
  • Shiyanovskii, S.V., O.D. Lavrentovich, T. Schneider, T. Ishikawa, I.I. Smalyukh, C.J. Woolverton, G.D. Niehaus, K.J. Doane (2005) Lyotropic chromonic liquid crystals for biological sensing application. Mol. Cryst. Liq. Cryst. 434:587-598.
  • Schneider, T., I.I. Smalyukh, T. Ishikawa, O.D. Lavrentovich, G.D. Niehaus, K.J. Doane, C.J. Woolverton (2005) Real-time microbe detection using a lyotropic chromonic liquid crystal biosensor. Phys. Rev. E 71:020702. PMID: 15783309. Full text
  • Doane, K.J., R. Bhattacharya, J. Marchant. (2002) Perturbation of ß1 integrin function using anti-sense or function blocking antibodies on corneal cells grown on fibronectin and tenascin: implications for corneal development. Cell Biol. Int. 26:131-144. PMID: 11846443. Full text
  • Howell, S.J., K.J. Doane (1998) Type VI collagen increases proliferation and prevents anti-ß1 integrin induced-apoptosis. Exp. Cell Res. 241:230-241. PMID: 9633532. Full text
  • Singh, B., K.J. Doane, G.D. Niehaus (1998) Ultrastructural and cytochemical evaluation of sepsis-induced changes in the rat pulmonary intravascular mononuclear phagocytes. J. Anat. 192:13-23. PMID: 9568557. Full text
  • Doane, K.J., S. Howell, D. E. Birk (1998) Identification and functional characterization of two type VI collagen receptors, a3ß1 and NG2, during avian corneal stromal development. Invest. Ophthal. Vis. Sci. 39:263-275. PMID: 9477982. Full text
  • Tsonis, P.A., K. J. Doane, K. Del Rio-Tsonis (1997) Expression of integrins during axolotl limb regeneration. Devel. Growth Differ. 39:9-14. PMID: 8188478. Full text
  • Doane, K.J., W-H. Ting, J.S. McLaughlin, D.E. Birk (1996) Spatial and temporal variations in extracellular matrix of periocular and corneal regions during corneal stromal development. Exp. Eye Res. 62:271-283. PMID: 8690037. Full text
  • Doane, K.J., D.E. Birk (1994) Differences in integrin expression during avian corneal stromal development. Invest. Ophthal. Vis. Sci. 35:2834-2842. PMID: 8188478. Full text
  • Doane, K.J., J.P. Babiarz, J.M. Fitch, T.F. Linsenmayer, D.E. Birk (1992) Collagen fibril assembly by corneal fibroblasts in three-dimensional collagen gel cultures: Small diameter, heterotypic fibrils are deposited in the absence of keratan sulfate proteoglycan. Exp. Cell Res. 202:113-124. PMID: 1511726. Full text
  • Doane, K.J., G. Yang, D.E. Birk (1992) Corneal cell-matrix interactions: Type VI collagen promotes adhesion and spreading of corneal fibroblasts. Exp. Cell Res. 200:490-499. PMID: 1572410. Full text
  • Doane, K.J., F.J. Roisen, F.J. Wilson (1992) The effect of nerve growth factor and dibutyryl cyclic AMP on cytoskeletal densities in cultured sensory ganglia. Tiss. Cell 24:367-378. PMID: 1321996. Full text
  • Doane, K.J., D.E. Birk (1991) Fibroblasts retain their tissue phenotype when grown in three-dimensional collagen gels. Exp. Cell Res. 195:432-442. PMID: 2070825. Full text
  • Birk, D.E., J.M. Fitch, J.P. Babiarz, K.J. Doane, T.F. Linsenmayer (1990) Collagen fibrillogenesis in vitro: Interaction of types I and V collagen regulates fibril diameter. J. Cell Sci. 95:649-657. PMID: 2384532. Full text
  • Loesser, K.E., K.J. Doane, F.J. Wilson, F.J. Roisen, S. Malamed (1986) Improved immunoelectron microscopic method for localizing cytoskeletal proteins in Lowicryl K4M embedded tissues. J. Histochem. Cytochem. 34:1477-1485. PMID: 3534078. Full text
  • Nagele, R.G., K.J. Doane, H. Lee, F.J. Wilson, F.J. Roisen (1984) A method for exposing the internal anatomy of small and delicate tissues for correlated SEM/TEM studies using polyethylene embedding. J. Microsc. 133:177-183. PMID: 6368835. Full text
  • Doane, K., R.A. McReynolds, F.J. Wilson (1983) Immunofluorescence localization of contractile proteins in the rat lung following bleomycin injury. Histochem. J. 15:82-88. PMID: 6187713. Full text

Education

Bachelor – Grinnell College, Grinnell, Iowa
Master – Rutgers University, New Brunswick, NJ
PhD – Rutgers & UMDNJ, New Brunswick, NJ
Postdoctoral – Robert Wood Johnson Medical School, Piscataway, NJ
Postdoctoral – Tufts Medical School, Boston, MA